High-definition spatial transcriptomics for in situ tissue profiling
Author
Summary, in English
Spatial and molecular characteristics determine tissue function, yet high-resolution methods to capture both concurrently are lacking. Here, we developed high-definition spatial transcriptomics, which captures RNA from histological tissue sections on a dense, spatially barcoded bead array. Each experiment recovers several hundred thousand transcript-coupled spatial barcodes at 2-μm resolution, as demonstrated in mouse brain and primary breast cancer. This opens the way to high-resolution spatial analysis of cells and tissues.
Department/s
- Familial Breast Cancer
- BioCARE: Biomarkers in Cancer Medicine improving Health Care, Education and Innovation
Publishing year
2019
Language
English
Pages
987-990
Publication/Series
Nature Methods
Volume
16
Issue
10
Links
Document type
Journal article
Publisher
Nature Publishing Group
Topic
- Medical Laboratory Technologies
Status
Published
Research group
- Familial Breast Cancer
ISBN/ISSN/Other
- ISSN: 1548-7105