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Performance audit has become increasingly common in the public administration and every western democracy has today an independent National Audit Office (NAO). However, although the external requirements may be met, the functioning of these agencies may be jeopardized by internal and more subtle cultural factors relating to the role of the performance auditor. In Sweden, a new NAO called Riksrevisionen was formed in 2003, with the merger of the two former agencies for national audit. In mergers, extensive resistance often arises in the horizontal dimension running between the merging organizations. In this case, however, the horizontal resistance was soon replaced by resistance in a vertical dimension, running between the three Auditor-Generals on the one hand and the auditors on the other hand. In this paper, we attempt to explain why this opposition occurred. A five year case study of the formation of the Swedish NAO has been conducted. Internal documents have also be scrutinized, as well as employee attitude surveys. It is concluded that the status of the auditor at the Swedish NAO has been hollowed out and hierarchy has increased. It is also noted that there has been a long-lasting dispute over what kind of audits should be conducted, where the Auditor-Generals have attempted to transform the previous audit approach with an approach more similar to compliance audit. Auditors have consistently objected, but some critics have been met with sanctions. In a recent parliamentary commission the argument raised by auditors was supported and the Auditor-Generals were requested to align. The study shows how the independence of the NAO is jeopardized with the de-professionalization of auditors and it also illustrates how professional and cultural factors may harm the outcome of public sector reform.